Metabolites (antibiotic) extraction from microbes?

More Ervina ..'s questions See All

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

Please take this survey?

Hello! I am a second year Clinical Psychology student at William James College. I have conducted a survey as part of my Doctoral requirement that measures the effectiveness of Cognitive Load...

12 February 2020 2,828 3 View

Extracting metabolites from actinomycetes?

extract metabolites from actinomycetes usually, culture broth was harvested by centrifugation at 4 000 r/min for 15 min. The supernatant was collected and added equal volumes of solvent. does...

10 March 2019 7,673 2 View

Extracting metabolites from actinomycetes?

extract metabolites from actinomycetes usually, culture broth was harvested by centrifugation at 4 000 r/min for 15 min. The supernatant was collected and added equal volumes of solvent. does...

02 March 2019 9,338 3 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

24 January 2019 5,810 1 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

31 December 2018 9,374 2 View

What happen If we add methanol to supernatant cell culture (bacteria)?

Will they form two layers ? (Organic and aqueous phase)?

10 November 2018 1,599 1 View

What is in the supernatant after centrifugation?

What happen If we add methanol to supernatant? Will they form two layers ? (Organic and aqueous phase) Or just one phase? Why we use methanol instead water for extraction? (antibiotic compound)

10 November 2018 8,439 4 View

What is the effect of autoclaving Casein?

Can we autoclaving casein at 121 C for starch casein agar preparation? Can we use denaturated casein for bacterial growth media?

28 October 2018 3,445 2 View

Why are my extracts (glucosamine) not completely soluble in water?

Hi, I have extracted glucosamine from lobsters shell, however my extracts are not soluble in water,Could you please tell me why and give me a suitable way to dissolve them in water? Thank you

04 May 2018 7,372 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Jasim Al-saadi

You must centrifuge first to collect supernatant and add the solvent to supernatant

Ervina ..

Thankyou very much jasim al-saadi for the answer

May i know whats the effect if we add the solvent first before centrifugation?

Jack Silver

I've needed to extract fermentation broths (microbes or fungal) that formed emulsions. Centrifugation helped to break the emulsions. It didn't mater that the cells were still there, they just settled at the bottom.