Can we centrifuge culture broth that already mix with solvent?
Or we must centrifuge first to collect supernatant and add the solvent to supernatant?
You must centrifuge first to collect supernatant and add the solvent to supernatant
Thankyou very much jasim al-saadi for the answer
May i know whats the effect if we add the solvent first before centrifugation?
I've needed to extract fermentation broths (microbes or fungal) that formed emulsions. Centrifugation helped to break the emulsions. It didn't mater that the cells were still there, they just settled at the bottom.
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