Metabolites (antibiotic) extraction from microbes?

Can we centrifuge culture broth that already mix with solvent? Or we must centrifuge first to collect supernatant and add the solvent to supernatant?

05 June 2019 923 3 View

Extracting metabolites from actinomycetes?

02 March 2019 9,275 3 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

24 January 2019 5,731 1 View

Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

31 December 2018 9,281 2 View

What happen If we add methanol to supernatant cell culture (bacteria)?

Will they form two layers ? (Organic and aqueous phase)?

10 November 2018 1,500 1 View

What is in the supernatant after centrifugation?

What happen If we add methanol to supernatant? Will they form two layers ? (Organic and aqueous phase) Or just one phase? Why we use methanol instead water for extraction? (antibiotic compound)

10 November 2018 8,295 4 View

Why are my extracts (glucosamine) not completely soluble in water?

Hi, I have extracted glucosamine from lobsters shell, however my extracts are not soluble in water,Could you please tell me why and give me a suitable way to dissolve them in water? Thank you

04 May 2018 7,295 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View