Extracting metabolites from bacteria with methanol?

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Extracting metabolites from bacteria with methanol?

If we are extracting metabolites from bacteria with methanol, which layer should we use (top or bottom layer)?

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What happen If we add methanol to supernatant cell culture (bacteria)?

Will they form two layers ? (Organic and aqueous phase)?

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What is in the supernatant after centrifugation?

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Faiq H S Hussain

In liquid- liquid extraction , there should be two immiscible solvents.What is the second solvent, if you have water, it is miscible with water. The top and bottom layers depend on the density of the solvents, top layer has lower density.

Abdel Rahman Mohammad Al Tawaha

Beautiful question and great answers

I wish you success in the service of humanity and science