How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to make PEDOT:PSS fluorescent?

Hello, I am spin coating thin films of PEDOT:PSS (Ossila, PH1000, ~50nm thick) on Si wafers and would like to make this thin films fluorescent. I have tried to dissolve fluorescent dyes in the...

05 January 2021 6,563 6 View

Does mysticism have a scientific basis?

Mysticism is often treated as the opposite of science. But is it? Please see Natural Laws and Mysticism in the Cosmic Cycle of Creation

25 July 2020 6,696 80 View

INEXT in R: Problems with processing data. Who can tell me, what I'm doing wrong?

Hello, I'm trying to compare two small datasets of Elasmobranchs landed by artisanal fisheries in Brazil via rarefaction curves that are based on sampling units, not abundances. Therefore, I...

02 April 2020 3,727 9 View

Could our fine-tuned universe have been created from a single fine-tuned quantum particle?

Cosmological explanations for our apparently fine-tuned universe are basically divided between a) a vastly huge multiverse of universes with varying fundamental force and mass constants, including...

21 February 2020 4,618 100 View

Small sample size in master thesis: What suggestions do you have how to statistically treat the data?

Dear everyone who might be able to help: Hello! I'm currently a student in marine science and I am writing my master thesis about Elasmobranchs and the big numbers that are being caught by...

19 February 2020 2,741 12 View

In SPSS post hoc results of a 2-way ANOVA, are "Multivariate tests" post hoc tests? If so, can the results be corrected by Bonferroni's correction?

Dear all, I am running a RM ANOVA with 2 independent variables (factor A with 3 levels, factor B with 5 levels). The AxB interaction is significant and I want to know using a post hoc test in...

13 March 2019 7,609 5 View

Can a SV40 polyA signal be inverted ?

Hi all. It may sound a bit silly but I was wondering whether a SV40 polyA signal could be inverted. For reasons, I have to introduce one in a plasmid of mine but I have only one unique...

13 November 2018 774 5 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

In CxG, where are collostructional preferences stored? Are they formal, functional or external properties of constructions?

Trying to apply key concepts from CxG to modality in English, I came across research by Boogaart (2009). He particularly looks at the modal verb 'kunnen' in Dutch and raises an interesting...

12 September 2018 3,687 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View