Dear Mehrdad,

I've encountered these before as well in R&D and, as you know, dsrB is not the most straightforward target. They're using a combination of multiple forward and multiple reverse primers to achieve greater coverage of SRBs.

Hope that helps clear that up and good luck!

JP

Jason Byron Perez

Sure, I realized that in my research thought the some papers. But The question is why the mix term is in forward primer? In other words, what is the actual difference between the mix and ordinary one's sequences?

Anyway thanks.

Dear Mehrdad,

Ah, I get your question now. I think it's just what they chose to indicate that the "mix" primer set contains multiple forward primers, which is 10 forward primers in the case of the one you mentioned.

Hope that helps!

JP

Jason Byron Perez

First of all, thank you very much for the fast reply!

To make sure that I got your point, I need to ask one more question in this case.

You meant in the mix primer, for instance, some forward primer's seuences get together and make only one combined primer sequence. Let's say, different nucluetides in differnt forward primers share their nucletides then generate the mix one. Am I right?

Thanks for help, and have a nice weekend.