Could you please tell me for what reasons we should use a cloned DNA segement as a standard to find out how much a certain gene expressed?

If you need an example, take a look the Quantitative PCR section in the following paper:

https://www.frontiersin.org/articles/10.3389/fmicb.2018.02787/full

" The cloned mcrA gene fragment of Methanosarcina barkeri was used as standard."

Best regards,

Mehrdad

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