Could you please tell me for what reasons we should use a cloned DNA segement as a standard to find out how much a certain gene expressed?
If you need an example, take a look the Quantitative PCR section in the following paper:
https://www.frontiersin.org/articles/10.3389/fmicb.2018.02787/full
" The cloned mcrA gene fragment of Methanosarcina barkeri was used as standard."
Best regards,
Mehrdad