I need to creat a standard curve for a qPCR assay by plasmid, after plasmid extraction, I run electrophorasis assay to check its quality. I visualized small nucleic acids in the lower layers beside the plasmid.

I firstly need to know what is that? Is it denatured DNA? RNA? Or degraged plasmid?

And second, is it interfered in my standard curve result?

By the way, second lane is 1 kb and third lane is sample. Another point the extraction has not been done from fresh culture.

Thaks a lot