What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
I want to simulate WRF for a 12*12 km domain size. I want to run LSM and BSM models with a 5 minutes time interval. My laptop specification is 16GB RAM and Intel i5-1235U 1.3 GHz processor. Can I...
13 July 2024 2,037 0 View
I am using the discrete fastener method for an LSF frame, and I want to consider the shear failure of rivets in my simulation. However, when I define lower and upper bounds for shear force, Abaqus...
06 June 2024 9,241 0 View
How can prepare Alginate Gelatin Chitosan hydrogel?
05 May 2024 6,229 2 View
Hello dears I want to import an LSF model from Tekla to Abaqus. Which type of file should the Tekla model be exported as?
30 April 2024 7,978 2 View
Does Gibbs 2 take into account both lattice and electronic contributions to the specific heat Cp ?
23 April 2024 2,280 0 View
Hi everyone, I'm conducting research on the impact of AI on workforce displacement in the logistics industry for my Bachelor's thesis. If you work in logistics and have experience with AI...
07 April 2024 4,394 1 View
Hi everyone, Hope you are all in good well and health. I am currently pursuing my degree in Bachelor of Science in Logistics. I am searching for reliable survey questions that I can use in my...
01 April 2024 2,499 2 View
Hello, Which are some AI tools that researchers can use in writing scientific paper?
23 February 2024 1,792 4 View
I have recently published a research paper about this actuator and I want know your idea about practical uses of this new actuator.
22 February 2024 10,071 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View