I was asked to provide the 16S rRNA gene primer's name.
I designed the primer for 16S rRNA gene with these details.
926F and 1392R primer Pair
I googled its name but I did not catch anything.
Please share your opinion!
If you designed your primers yourself, as you claim .. how would you find reference for that ?
And if you took these from some article or somewhere, why not go back there and provide appropriate citation.
By the way if its 925f and 1392r, they are published primer sets.
I'm not sure what you are asking. Do you want to know if these are already "named" and published? Don't rely on Google! Do a search for the 16S rDNA in your organism in a scholarly website (PubMed, Web of Science, etc.). And yes, if your mean 925F and 1392R, these are already published and widely used.
Katie A Burnette Shadman Sadiq
Thanks for reply. Yeah it is universal primer.
Sometimes I heard come people say V3 V4 variable region 3 and variable region 4, respectively. Or maybe the primers have special name! I looked it up I did not fine any answer.
Abhijeet Singh yes, it is widely used! but I used stagger + barcode when I chose the primer sets and designed in terms of primer dimer and self-complementary that's why I said 'I designed'. Anyway, thanks for the reply!
I have a relevant question:
If it is possible, could you please tell me how we can know which part/ region of 16S rRNA gene are universal?
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