Dear researchers,

As you know, it might be some ambiguity nucleotides in primer oligos appeared. For example, in my case, which I took the forward and reverse primers from a paper, there are some of them.

Froward: GGTGGTGTMGGDTTCACMCARTA

Reverse: CGTTCATBGCGTAGTTVGGRTAGT

Now, I would like to know, what a exact nucleotide among the normal nucleotides (A, G, C and T) places inseated of the ambiguity ones (M, R, V, D and B) when a primer making center generates them?

Thanks in advance

Mehrdad Rabiei

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