Dear researchers,
As you know, it might be some ambiguity nucleotides in primer oligos appeared. For example, in my case, which I took the forward and reverse primers from a paper, there are some of them.
Froward: GGTGGTGTMGGDTTCACMCARTA
Reverse: CGTTCATBGCGTAGTTVGGRTAGT
Now, I would like to know, what a exact nucleotide among the normal nucleotides (A, G, C and T) places inseated of the ambiguity ones (M, R, V, D and B) when a primer making center generates them?
Thanks in advance
Mehrdad Rabiei