Mehrdad Rabiei

10 Questions 16 Answers 0 Followers

Questions related from Mehrdad Rabiei

What does the "mix" term say in dsrB forward prime?

Hi, In the fiollowing paper I would like to know what is the meaning of mix in dsrB forward primer? Diversity analysis of sulfite- and sulfate-reducing microorg... DSR1762F2 DSR1728FmixB dsrB...

11 November 2019 8,701 4 View

Why some microbial terms and details are not present in the textbooks?

I was trying to find alpha, beta and gamma diversities in the Brock biology of microorganisms book, but there is no such info in. Similarly, I could not find evenness and richness phrases. I have...

01 January 1970 1,940 8 View

16S rRNA Gene Primer's Name

I was asked to provide the 16S rRNA gene primer's name. I designed the primer for 16S rRNA gene with these details. 926F and 1392R primer Pair I googled its name but I did not catch anything....

01 January 1970 8,200 4 View

Archaea domain in Taxonomy Bar Plot

In taxonomy bar plot, I have some Archaea domains while I used bacterial 16S rRNA primer sets. How possible to justify it?

01 January 1970 1,177 1 View

Normalisation for Microbial Ecology Data

I have two treatment groups of sample plus a group control. Each treatment and control have own three replicates. I am using Qiime2 for data analysis. I am in dilemma to the following ways below....

01 January 1970 4,531 4 View

Reference sequence vs. reference taxonomy

Hello Dear Friends, Hope you are doing well! I have a question regarding the two phrases: 1. reference sequence 2. reference taxonomy What is the difference between them? What makes them...

01 January 1970 2,857 1 View

What is the bright band in the third lane on agarose gel after plasmid extraction?

I need to creat a standard curve for a qPCR assay by plasmid, after plasmid extraction, I run electrophorasis assay to check its quality. I visualized small nucleic acids in the lower layers...

01 January 1970 3,252 1 View

In Microbiology: Why a cloned DNA segment is used to determine a gene expression?

Could you please tell me for what reasons we should use a cloned DNA segement as a standard to find out how much a certain gene expressed? If you need an example, take a look the Quantitative PCR...

01 January 1970 8,761 4 View

Ambiguity Nucleotides in Primer making: How to deal with them in generating?

Dear researchers, As you know, it might be some ambiguity nucleotides in primer oligos appeared. For example, in my case, which I took the forward and reverse primers from a paper, there are some...

01 January 1970 4,577 2 View

How to find a PCR amplicon length by a primer set?

I could not find the PCR amplicon length by a primer set mentioned in a paper. I googled and followed the references, but not worked. Have you got any suggestion?

01 January 1970 7,759 5 View