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02 January 2021 7,172 4 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

Which statistical analysis could be used in stomach content data?

Hi everybody, I'm looking at stomach content of a damselfish, to analyze the diet in two sites. I'm going to use the numerical proportion of the prey items that I found in each stomach. In each...

17 November 2020 4,967 3 View

What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

Recommend antibodies that works in paraffin sections for synaptic and astrocytes markers in rat samples?

Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...

12 August 2020 5,382 2 View

Innovation, Ideas permeability. Which factors make an idea to be commercialized?

There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...

13 July 2020 7,807 12 View

What is the minimum viral load that can be detected by TEM or SEM?

Imagine SARS-COV-2 in a surface to be recovered with an hyssop .

08 July 2020 1,193 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

What order is the change in the shift or intensity of a XRD pattern when there is an impurity in the crystal lattice?

I doped a fluoride perovskite LiMgF3 with different concentrations of Cr (0.25%mol and 0.5%mol). The XRD pattern of LiMgF3 shows the highest peak at 38.9847, but LiMgF3:Cr 0.25%mol shows this peak...

06 July 2020 5,630 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

How to measure cosine, abbe, deadpath errors?

Dear sir, How to measure cosine, abbe, deadpath errors. I know cosine error, abbe error, deadpath error occurs in laser measurement. But how i can identify the cosine error value. Is any separate...

03 February 2021 4,878 4 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

How to apply graphene oxide on an gold electrode?

Dear Fellows, now it is the time to push graphene applications further in terms of easy and low cost production surroundings. Thus I recently dived in the materia and I am facing tons of papers,...

02 February 2021 6,284 2 View

Which method is preferable for cutting the nanocomposites?

Which of the methods like water jet cutting, diamond cutting, laser cutting, etc. is more suitable to cut the carbon nanotubes reinforced epoxy composites into test sample to ensure the clean...

29 January 2021 1,354 5 View