What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

Lyophilized Antibodies for Microfluidic Device?

I am using monoclonal lyophilized antibodies for a microfluidic chip which I would like to be stored at room temperature. What are the easiest methods to reactivate these antibodies for...

31 March 2018 8,941 2 View

What the best method for Research about Fruit Flies (Bactrocera spp)?

Entomology, Phytopathology, Integrated Pest Management

05 March 2017 6,708 14 View

How do i measure body size/image incongruence?

Hi. I am looking for a way to measure body size or body image incongruence. i would like to try and measure the differnece between actual and perceived body size. i was wondering if anyone knows...

11 December 2016 6,056 8 View

Are there any specific antibodies or markers for M phase of cell cycle?

07 December 2016 4,014 3 View

Can anyone recommend a good dissociation questionnaire, other than the DES?

i am looking for a good dissociation questionnaire, other than the DES. i would like something that had been validated and has been cited often.

10 November 2015 1,929 7 View

Why do dividing RPTECs show two different cluster morphologies?

We are currently culturing immortalized renal proximal tubules (RPTEC/TERT1) at passage 1. The cells are adherent, and so grow in clusters, but strangely, we observe two different types of...

24 June 2014 5,073 2 View

Does leaving (dilute) trypsin in with the cells during passage slow their growth?

I am growing hCMEC/d3 cells in Lonza EGM media with Bulletkit supplements. The passage protocol I use calls for just directly adding the resuspended cells, trypsin (.25% in EDTA) and all, to the...

03 March 2014 6,717 5 View

Apakah mahasiswa bisa dilibatkan dalam project ini?

saya mahasiswa tingkat akhir yang ingin mencari project penelitian untuk tugas akhir , saya adalah mahasiswa fisika saya tertarik dengan remote sensing pemetaan dan tomografi, mungkin jika...

01 January 1970 7,177 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How do I apply continuity boundary condition at the edge using form assembly in comsol?

The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...

05 February 2021 3,459 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View