We would like to phosphorilate enzymatically a synthetic peptide derived from histone H3. Its sequence is QTARKSTGGK. Is there any commercial kinase kits that could be used for this purpose? I have done some research on the Internet, and I only found kinase assays that measure the activity of the kinase. Is there any kit with an enzyme that could be used for global phosphorylation on peptides, as there are enzymes used for digestion or deimination like PADI enzymes? I know that many kinase exists each of them specified for certain substrates. But maybe there are some commercially available kinase specially used for phosphorylation of histones or histone peptides. If you have any information about that, please let me know :)
Kinases are specific for certain polypeptide sequences they can recognize. This said you should ask Thermo Fisher Scientific (https://www.thermofisher.com/it/en/home.html) if they have a specific kinase for your sequence.
Good luck.
As said above, these sites (S, T?) are substrates for specific kinases. For example PKC can phosphorylate the S10 of H3. This said, if you perform a phosphorylation assay with purified active PKC, ATP and your substrate, along with positive and negative controls and reveal the phosphorylation with an antibody recognizing phosphorylated PKC substrate it should work,
I hope this helped,
Dear all!
Thank you very much for your answers! We would like to further investigate the peptides with mass spectrometry so the efficiency of the phosphorilation would be proven by mass spectrometry. Thank you for all of your advice, PKC seems to be a good choice then :)
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