Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Can we do electrophoresis of RNA from baker's yeast by vertical electrophoresis (using acrylamide gels)?

I want to do an electrophoresis of RNA from baker's yeast. However, our lab only has a vertical electrophoresis, while usually electrophoresis of nucleic acid (RNA or DNA) use horizontal...

29 November 2020 7,938 5 View

What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

Lyophilized Antibodies for Microfluidic Device?

I am using monoclonal lyophilized antibodies for a microfluidic chip which I would like to be stored at room temperature. What are the easiest methods to reactivate these antibodies for...

31 March 2018 8,941 2 View

What the best method for Research about Fruit Flies (Bactrocera spp)?

Entomology, Phytopathology, Integrated Pest Management

05 March 2017 6,708 14 View

How do i measure body size/image incongruence?

Hi. I am looking for a way to measure body size or body image incongruence. i would like to try and measure the differnece between actual and perceived body size. i was wondering if anyone knows...

11 December 2016 6,056 8 View

Are there any specific antibodies or markers for M phase of cell cycle?

07 December 2016 4,014 3 View

Can anyone recommend a good dissociation questionnaire, other than the DES?

i am looking for a good dissociation questionnaire, other than the DES. i would like something that had been validated and has been cited often.

10 November 2015 1,929 7 View

Why do dividing RPTECs show two different cluster morphologies?

We are currently culturing immortalized renal proximal tubules (RPTEC/TERT1) at passage 1. The cells are adherent, and so grow in clusters, but strangely, we observe two different types of...

24 June 2014 5,073 2 View

Does leaving (dilute) trypsin in with the cells during passage slow their growth?

I am growing hCMEC/d3 cells in Lonza EGM media with Bulletkit supplements. The passage protocol I use calls for just directly adding the resuspended cells, trypsin (.25% in EDTA) and all, to the...

03 March 2014 6,717 5 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Which test to use check normality for count data if I have 3 treatments and one dependent variable?

I am working on a experimental design. I have three treatments(three temperatures) . I have 18 fish under each sample and after a desired time I will check if there is any variation in their...

01 January 2021 9,167 3 View

What is the preferable incubation period in injecting ethanol into the duck eggs for antiteratogenic studies?

I have been working on the anti-teratogenic activity of a plant extract towards ethanol induced duck embryos however, many studies use the 14 days incubation period and then remove the embryo for...

17 November 2020 4,701 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

What is the protocol of eggs sampling from an eggs production unit ?

Dear researchers greetings, I'm working on eggs quality for my Ph.D thesis and I want to know what is the protocol for eggs sampling from a production unit. The main questions I have are the...

13 October 2020 9,716 7 View

In egg albumin denaturation assay, how to measure absorbance using spectrophotometer accurately ?

I am doing the egg albumin denaturation test to evaluate the anti-inflammatory activity of my SCE. After keeping the reaction mixtures in the water bath, the egg albumin gets denatured while...

20 August 2020 9,686 1 View

How do Trichogramma parasitoids find their hosts?

I am planing an experiment to investigate the dispersion ability of Trichogramma dendrolimi under semi-field conditions. As far as I know Trichogramma uses kairomones for host finding but are...

29 June 2020 4,262 1 View