What could be the possible reasons for obtaining a precipitate after dissolving fromazan crystals in acidified isopropanol solution in MTT assay?

Hi, I am running an MTT assay where i use an acidic solution of isopropanol, HCl and Triton X -100 to dissolve formazan crystals. I have tried using DMSO before but failed to dissolve crystals...

29 December 2019 6,234 2 View

How do I calculate IC50 of a drug towards OCI AML2 cell line?

Hi, I am in the process of learning to calculate IC50 values of OCI AML2 cells for Cytarabine. I have taken the drug concentrations as 0.01 uM to 50uM. My question is while calculating IC50 is it...

18 December 2019 3,639 3 View

What is the appropriate reagent to dissolve formazan crystals in an MTT assay?

Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...

03 December 2019 8,862 3 View

What methods can be used to measure suitability of an ecosystem based on plankton population?

I intend to understand how sustainable an aquatic ecosystem is based on its plankton population. Can anyone help me with suitable methods available?

19 February 2019 3,838 13 View

Would anyone know of a scale that maps paternal involvement or attachment/parenting practices? esp. one that can be used in a non-western context?

03 August 2016 950 6 View

To analyse thin filem using SEM which substrate is preferred? What should be the size and thickness of it?

For forming thin film of metal doped nano TiO2, glass or Silicon surface is preferred and of what size?

26 April 2016 6,656 6 View

Does anyone know of a short questionnaire to assess child attachment or parenting style?

Child attachment related brief tools

30 July 2013 9,530 12 View

How do I differentiate monocytes into a phenotype similar to Tumor-associated macrophages (for breast cancer)?

Would a good approach be to use supernatants from various breast cancer cell lines and differentiate these CD14+ monocytes from healthy donor blood into macrophages in their presence for 10-14...

01 January 1970 4,788 2 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View