Manasi Nagare

5 Questions 6 Answers 0 Followers

Questions related from Manasi Nagare

What could be the possible reasons for obtaining a precipitate after dissolving fromazan crystals in acidified isopropanol solution in MTT assay?

Hi, I am running an MTT assay where i use an acidic solution of isopropanol, HCl and Triton X -100 to dissolve formazan crystals. I have tried using DMSO before but failed to dissolve crystals...

30 December 2019 6,289 2 View

How do I calculate IC50 of a drug towards OCI AML2 cell line?

Hi, I am in the process of learning to calculate IC50 values of OCI AML2 cells for Cytarabine. I have taken the drug concentrations as 0.01 uM to 50uM. My question is while calculating IC50 is it...

19 December 2019 3,695 3 View

What is the appropriate reagent to dissolve formazan crystals in an MTT assay?

Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...

04 December 2019 8,925 3 View

Drug resistant AML cell lines?

I am working on Acute myeloid leukemia (AML) bone marrow derived Mesenchymal stem cells and will be studying their interactions with AML cells. For standardization process i need to work with drug...

05 November 2019 4,486 5 View

How to perform co-culture experiments?

Hi, I want to check the effect of bone marrow derived mesenchymal stem cells on the proliferation of leukemic blasts as well as standard cell lines in the presence and absence of standard...

18 February 2019 7,950 5 View