Hello, I need to do a Total Antioxidant Capacity test, but unfortunately we don't have Trolox. We are testing the coffee extract so what can we use as the standard solution instead of Trolox?
If you don't have trolox you can go for ascorbic acid as a standard. I have done several scavenging assays such as ABTS, DPPH, total antioxidant capacity, lipid peroxidation and beta carotene bleaching assay with ascorbic acid has been used as a standard.
Throlox can be used as a control in experiments examining the effects of antioxidants on biological systems. It can also be used as a standard in analytical methods to quantify the levels of antioxidants in samples. Furthermore, Throlox has been used in cellular and animal studies to evaluate its potential therapeutic effects on various conditions, such as oxidative stress and inflammation.
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