Problem is in running Gaussian 09 job (Windows). After submitted the job is immediately deleted. The check-route says 'file is not specified'. Why ?

I am using Gaussian 09 (windows). The problem has generated over last few days. Whenever I submit the job, it is immediately deleted with no error message and the output file is empty. The...

10 July 2020 2,604 1 View

How much volume of assay buffer should I use to lyse my cells for pyruvate kinase assay using the Abcam kit?

The kit suggests, 'tissues or cells can be extracted with 4 volumes of the assay buffer'. This statement appears quite cryptic as it doesn't tell me 4 volumes of what? For instance, I'm going to...

05 September 2019 9,254 3 View

How to get the t-values for standardized regression weights in SEM?

Query 1: How to get the t-values for standardized regression weights in SEM? AMOS output provides CR values cosponsoring to the unstandardized estimates only. In journal papers usually the...

21 December 2018 5,691 11 View

For better creep strength, which microstructure should be prefered in a superalloy ?

Bimodal grains with bimodal precipitates or bimodal precipitate with coarse grain, For better creep strength will bimodal grain size plays a vital role or bimodal ppt size plays the key role for a...

05 August 2016 2,151 19 View

Can somebody help me with the Biso parameters in the Rietveld refinement using Full Prof software???

I have been trying to do do the Rietveld refinement using full prof software. I have achieved a chi2 value of about 1.9. But while i am trying to refine Biso, some of the parameters are going...

05 April 2016 9,251 5 View

Is the chemical synthesis of peptides necessary?

I am working on generating bioactive peptides from a natural source. I have read a few articles where people chemically synthesize the peptides after they have identified it in their bioactive...

10 March 2015 3,720 8 View

Is it rational to adopt a cover depth corresponding to the maximum moisture penetration depth in concrete?

29 September 2014 7,727 11 View

Can anyone recommend a pressure cell for transmission spectroscopy on polymer thin film under pressure?

I am in search of a pressure cell with optical (mostly visible wavelength regime) windows, where I can place a soft polymer thin film and squish it under controlled calibrated pressure for...

04 December 2012 7,150 9 View

Does it make sense to think of a completely empirical Physics dispensing altogether with the presiding Metaphysics ?

01 January 1970 7,367 60 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View