I have tried with ethidium bromide and stains-all for staining but not getting any band after one day of incubation. I tried with both Native and Non-Native gel.
It depends on the amount of poly dt and its length. Et Br is quite insensitive particularly with single stranded dna.
In PAGE gels silver staining is more sensitive.
If the amounts are large (0.3ug) the UV shadowing where you illuminate the gel with UV light (about 254nm) with a fluorescent tlc plate or x-ray fluorescent plate and look for dark area where the dna is found. The sybr range of dyes are more sensitive than EtBr but some are better than others for ssDNA detection
Thank you very much Paul Rutland sir for your suggestion. I have tried with 1 nmole of dT oligo. Sure, I will try silver staining and will let you know.
That is quite a lot of oligo. you may want to consider methylene blue staining of the oligo run in denaturing PAGE as detailed in this protocol:
Methylene Blue Oligonucleotide Staining & Quality Analysis (genelink.com)
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