There are several ways that can be done to increase rhamnolipid products through biochemical synthesis pathways. In the fermentation pathway, controlling pH is the key in obtaining high yields, in addition to the concentration and ratio of substrate settings. we can predict the equilibrium constant for rhamnolipid production, and shift the thermodynamic equilibrium to the left more, to increase the yield. the rhamnolipid yield was increased from 24.06 to 28.8 g/l simply by controlling the pH during batch fermentation, which was further increased to 70.56 g/l using pH-controlled fed-batch fermentation

High yielding strains of bacteria such as Pseudomonas aeruginosa are rare to come by naturally. However, by careful metabolic and genetic manipulations, significant improvement in yield of rhamnolipid has been reported. My strain of Pseudomonas aeruginosa does not produce rhamnolipid, but a glycolipopeptide biosurfactant, and is currently undergoing a lot of genetic manipulations. At the moment, I am working on the use of atmospheric and room-temperature plasma (ARTP) mutagenesis to improve yield. This was necessary after a series of optimization experiments on major and trace nutritional, as well as environmental and cultural requirements of the bacterial strain.