20 January 2022 1 4K Report

I am currently work with antifungal study. I will be doing spore counting by using hemocytometer. I have searched some info that trypan blue can be used to differentiate live and dead cells. However, is this worked on fungal spores too? Any reference or guidelines about this? Is there any other methods can be used for spore counting?

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Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?

Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?

16 March 2023 4,105 0 View

How to do anti-sporulation test in microtiter plate?

I am studying antifungal drug, but I only have limited drug sample. So I am planning to run the experiment in microtiter plate. Is there any manual, guidelines or reference about this?

16 January 2022 7,585 0 View

How to do fungus sample preparation for observation under scanning electron microscope (SEM)?

I have a fungus sample (Fusarium sp.) and I need to observe the mycelial of Fusarium sp. under SEM. Any guidelines on fungus sample preparation and examination under SEM? What is the most suitable...

10 November 2021 950 5 View

What are the ways to produce high yield of rhamnolipid from Pseudomonas aeruginosa?

I am given a task to extract rhamnolipid from P. aeruginosa. What are the ways or precautions can be done to produce high yield of rhamnolipid?

28 October 2021 7,071 2 View

What is the effect of phytopathogenic fungi, Fusarium spp. towards agricultural crops?

Any related studies or references about this? Common species of Fusarium found in agricultural crops Factors or how of Fusarium spp. grow / Where does the infection of Fusarium come from? Viral...

05 October 2021 2,295 11 View

How do you confirm a RNA and its role on rRNA maturation?

You have discovered a novel species of short RNA expressed only in hepatocytes but not in other cell types. It is partly complementary to excised portion of rRNA. You hypothesize that this RNA is...

10 April 2021 1,266 1 View

Can we use mapping technique?

I have a doubt regarding whether we adopt mapping technique in social science research, were the population list is not available?

04 March 2021 1,519 3 View

How can we assay AP endonuclease involved in DNA repair?

I am cloning endonuclease in pET vector

06 October 2019 985 1 View

How can we assay endonuclease?

I am cloning a gene whose protein product is endonuclease what enzyme assays should I do after successful purification of the protein

29 September 2019 493 7 View

Error in plotting gain versus frequency in CST,solution?

When I do the evaluation in postprocessing I get the error- Template based post-processing result "Gain (IEEE),3D,Max. Value (Solid Angle)": Error in calling "Evaluate1D" function (No calculated...

18 December 2018 2,425 6 View

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