I am studying antifungal drug, but I only have limited drug sample. So I am planning to run the experiment in microtiter plate. Is there any manual, guidelines or reference about this?
Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?
16 March 2023 4,105 0 View
I am currently work with antifungal study. I will be doing spore counting by using hemocytometer. I have searched some info that trypan blue can be used to differentiate live and dead cells....
19 January 2022 3,591 1 View
I have a fungus sample (Fusarium sp.) and I need to observe the mycelial of Fusarium sp. under SEM. Any guidelines on fungus sample preparation and examination under SEM? What is the most suitable...
10 November 2021 950 5 View
I am given a task to extract rhamnolipid from P. aeruginosa. What are the ways or precautions can be done to produce high yield of rhamnolipid?
28 October 2021 7,071 2 View
Any related studies or references about this? Common species of Fusarium found in agricultural crops Factors or how of Fusarium spp. grow / Where does the infection of Fusarium come from? Viral...
05 October 2021 2,295 11 View
You have discovered a novel species of short RNA expressed only in hepatocytes but not in other cell types. It is partly complementary to excised portion of rRNA. You hypothesize that this RNA is...
10 April 2021 1,266 1 View
I have a doubt regarding whether we adopt mapping technique in social science research, were the population list is not available?
04 March 2021 1,519 3 View
I am cloning endonuclease in pET vector
06 October 2019 985 1 View
I am cloning a gene whose protein product is endonuclease what enzyme assays should I do after successful purification of the protein
29 September 2019 493 7 View
When I do the evaluation in postprocessing I get the error- Template based post-processing result "Gain (IEEE),3D,Max. Value (Solid Angle)": Error in calling "Evaluate1D" function (No calculated...
18 December 2018 2,425 6 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View