How to do fungus sample preparation for observation under scanning electron microscope (SEM)?

More Ria Ferdina's questions See All

Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?

16 March 2023 4,104 0 View

Can trypan blue differentiate live and dead spores?

I am currently work with antifungal study. I will be doing spore counting by using hemocytometer. I have searched some info that trypan blue can be used to differentiate live and dead cells....

19 January 2022 3,591 1 View

How to do anti-sporulation test in microtiter plate?

I am studying antifungal drug, but I only have limited drug sample. So I am planning to run the experiment in microtiter plate. Is there any manual, guidelines or reference about this?

16 January 2022 7,584 0 View

What are the ways to produce high yield of rhamnolipid from Pseudomonas aeruginosa?

I am given a task to extract rhamnolipid from P. aeruginosa. What are the ways or precautions can be done to produce high yield of rhamnolipid?

28 October 2021 7,071 2 View

What is the effect of phytopathogenic fungi, Fusarium spp. towards agricultural crops?

Any related studies or references about this? Common species of Fusarium found in agricultural crops Factors or how of Fusarium spp. grow / Where does the infection of Fusarium come from? Viral...

05 October 2021 2,295 11 View

How do you confirm a RNA and its role on rRNA maturation?

You have discovered a novel species of short RNA expressed only in hepatocytes but not in other cell types. It is partly complementary to excised portion of rRNA. You hypothesize that this RNA is...

10 April 2021 1,266 1 View

Can we use mapping technique?

I have a doubt regarding whether we adopt mapping technique in social science research, were the population list is not available?

04 March 2021 1,519 3 View

How can we assay AP endonuclease involved in DNA repair?

I am cloning endonuclease in pET vector

06 October 2019 985 1 View

How can we assay endonuclease?

I am cloning a gene whose protein product is endonuclease what enzyme assays should I do after successful purification of the protein

29 September 2019 493 7 View

Error in plotting gain versus frequency in CST,solution?

When I do the evaluation in postprocessing I get the error- Template based post-processing result "Gain (IEEE),3D,Max. Value (Solid Angle)": Error in calling "Evaluate1D" function (No calculated...

18 December 2018 2,425 6 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 718 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Divya Jain

Ria Ferdina Hope this will help you out. Please find the attached PDF.

William Letter

I have always found that speaking with the person in charge of the SEM , who has received formal training in its use and operation, is very beneficial. Their experience in sample preparation may provide you with a quick answer to your question.

Ludwig Gruber

Cryo-Prep might also a good approach, if available.....

Irshad Ahmad

Please let me know that in my email ID [email protected].

Ca Dr. Gaurav Bhambri

Dear Ria Ferdina Kindly check...

Article Scanning Electron Microscopy (SEM) Protocols for Problematic...