Good evening everyone.
do you have references related to the type of collagenase for cell culture, such as the optimum temperature, the duration of the optimum incubation time, and the mechanisms in breaking down the protein in detail?
There are several types of Collagenases available. In our lab we use Type III for dissociating cells from spleen. The activity of collagenase depends on the type, purity level and other modifications done by different manufacturers. The following links could provide you with a better understanding for the same. Good luck
Article Effective cell collection method using collagenase and ultra...
https://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/collagenase-guide.html
http://www.worthington-biochem.com/cls/default.html
As Ramachandramouli said, the conditions would be different for different enzyme preparations. A general overview of tissue dissociation enzymes (including collagenases) can be found in the following link.
http://www.conversantbio.com/blog/8-types-of-enzymes-for-tumor-cell-dissociation
http://www.worthington-biochem.com/CLSAFA/default.html
This company is leader in enzymes and collagenases, you can get plenty of information from their site, they are pioneers in this area
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