Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

How to do molecular dynamic simulation?

I want to carryout molecular dynamic simulation of some amphiphilic molecules to study their assembling behavior. My particular interest is in the lamellar bilayer arrangements. Someone, please...

16 May 2019 9,997 7 View

Do states, inputs, and outputs used in observer have to be the same as states, inputs, and outputs used in controller?

I'm designing an extended Kalman filter observer in a control system. In the control system, there is an MPC controller. Do states, inputs, and outputs used in observer have to be the same as...

17 March 2019 4,114 3 View

How to apply thermal and structural loading for each time step in thermal-structural analysis in ANSYS APDL?

I'm simulating an experiment in which a wood beam is submitted to thermal and structural loads at the same time. The beam is put in a furnace where tempeature rises according to ISO curve and at...

18 June 2018 8,802 9 View

What are the types of collagenase and how does it work?

Good evening everyone. do you have references related to the type of collagenase for cell culture, such as the optimum temperature, the duration of the optimum incubation time, and the mechanisms...

15 May 2018 8,618 3 View

How to control smoke generation in Phenol Formaldehyde resole resin in coated abrasives?

I would like to control the smoke generation in PF (Resole) resin formulation for coated abrasives. Can any one suggest the technique...

06 May 2018 3,805 2 View

Please tell me any one what is the meaning of G99 in ASTM G99 pin on disc wear test standard ?

Please tell me any one what is the meaning of G99 in ASTM G99 pin on disc wear test standard ?

20 May 2017 2,722 3 View

Detecting specific antigen to an unknown Antibody?

If I have an antibody enriched in a sample and I would like to know which antigen to employ, what techniques can I use? Do you think methods such as co-IP coupled with mass spec analysis is the...

10 January 2013 4,683 17 View

Is there a minimum optimal diameter for neural stimulating electrodes?

With the 1.27mm diameter of DBS electrodes often leading to the development of scar tissue, and evidence that electrodes with diameters near the size of neurons, approximately 30 microns, are less...

26 March 2020 1,782 3 View

Which companie's PD-L1 is better to work with for immunohistochemistry?

CST and DBS PD-L1 is not working for me. Can any one suggest a better option?

29 January 2020 8,344 10 View

Can tDCS and DBS rewire and reorganize the brain networks?

Is tDCS able to change the structure, wiring and the organization of the brain neural networks? How about DBS? How long does it take?

18 January 2019 5,272 2 View

Automated robotic genomic DNA extraction protocol for DBS ?

Can anyone share me a detailed genomic DNA extraction protocol for QIAcube? Also let know if anyone uses automated DNA extraction from DBS sample?

31 December 2018 891 3 View

Can tDCS and DBS rewire and reorganize the brain networks?

Is tDCS able to change the structure, wiring and the organization of the brain neural networks? How about DBS? How long does it take?

31 December 2018 6,635 1 View

Is it okay to use expired Whatman 903 Filter Papers for PCR?

I am wondering if it would affect DNA quality/yield if one uses dried blood spots (DBS) from expired Whatman 903 filter papers. Why does the manufacturer put an expiry date on the filter paper?

09 October 2018 6,552 0 View

Experience or expertise with the rodent catalepsy model for assessing EPS liability of Dopamine antagonists?

Can you recommend references? Any suggestions for comparing EPS liability for D2/D3 antagonists that are pGP substrates?

08 September 2018 3,771 1 View

Why GPi-DBS patients report QoL Measures as high as STN-DBS patients even if they experience less motor improvement?

Quality of Life after DBS in PD Recent comparative studies between PD patients who underwent either GPi or STN DBS report a small but significant difference in motor outcomes (10--15%) STN>GPi....

05 June 2018 7,710 0 View

Does anyone know of Alternatives to Whatman FTA cards? OR a way in which to simply preserve Nucleic acids on DBS (dry blood spots)?

Looking for a way to preserve nucleic acids on DBS. Currently using 3MM filter paper which is great for storing blood samples but we need a way to ensure quality long term.

05 June 2018 453 0 View

Is DBS effective on facial expressions?

04 May 2018 3,708 0 View