Vikas Kumar Patel

11 Questions 62 Answers 0 Followers

Questions related from Vikas Kumar Patel

What mistake I did during Gibson assembly of 5 fragments, I did not found transformed colonies. Can anyone suggest me?

Dear all, I did assembly of of five different DNA fragments having length (A= 975 bp, B=950 bp, C= 748 bp, D=1022 bp, Vector backbone= 1790 bp). The concentrations of these gel purified DNA...

10 October 2017 3,487 7 View

How to design in frame cloning construct for two genes that I can get both individual functional proteins from a single polycistronic RNA?

I am willing to design a suitable construct of two genes in a single reading frame, both of which could express with same efficiency under the regulation of a common promoter and RBS. I have to...

06 June 2016 4,603 4 View

I have to amplify a DNA sequence of about 14 kb from a plasmid, please suggest me which Taq polymerase should be used to get proper amplification?

I want to make a marker free expression vector, therefore, I am replacing the antibiotic resistance site of a vector with reporter genes, To achieve my target i have to get amplification of 14 kb...

02 February 2016 7,323 4 View

Is it possible to clone the PCR product directly into an expression vector?

I want to express an enzyme in cyanobacteria. For that I amplified the coding sequence of this enzyme from another organism. Is it possible to clone this PCR product directly into an expression...

03 March 2014 7,646 20 View

Can anyone suggest me a manual protocol for DNA purification from agarose gel?

I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...

01 January 2014 6,124 42 View

Can anyone suggest to me why I am not getting amplification of the gene?

I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...

08 August 2013 8,851 28 View

How do I discard the Agarose gel (containing ETBR) after electrophoresis?

Since ETBR is a carcinogenic chemical, what would be an appropriate way to discard the agarose gel containing ETBR?

08 August 2013 1,932 27 View

May i use the SALTS OF Ni2+ at the place of Ni2+ for inducing the promoter activity

I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?

08 August 2013 2,967 1 View

Is it possible to develop intergeneric somatic hybrids between bacteria?

If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it...

07 July 2013 8,917 0 View

How to develop the competency in filamentous organism?

How can we develop the competency in filamentous organism like Nostoc and Anabaena spp.?

12 December 2012 6,290 3 View

Suitable cloning vector for cloning gene construct of 4 kb in cyanobacteria

hi everyone can anyone help me that, which cloning vector will be more suitable for cloning the gene construct of about 4 kb in cyanobacteria

08 August 2011 3,898 1 View