How to chose the best trendline option for biological data?

03 April 2018 2,652 3 View

No title

31 December 2017 1,442 3 View

Do plants have similar PI3K-Akt signaling pathway?

09 October 2017 3,576 1 View

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

07 August 2017 2,098 13 View

What's the best way to determine the insertion site of a transgene in an Arabidopsis?

Can I use LB and RB boarder primers to amplify the either sides of the trans-gene (cassette)? For this does it necessary to check the copy number of the transgene first?

07 August 2017 5,940 3 View

How TOR regulate the autophagy in plants (Arabidopsis)?

31 December 2016 6,924 3 View

What is next, after gathering data from molecular biology experiment? How do you approach data analysis and interpretation?

31 December 2016 1,585 3 View

How to make a cDNA library for protein protein interaction (Y2H)?

07 August 2016 9,340 3 View

How over expression of receptor protein affects in vivo (plants)?

How over expression of receptor protein affects in vivo (plants)? (biochemically) using ectopic promoters / interact protein etc.....

06 July 2016 3,963 1 View

How to compare sample means reference to specific sample mean?

What is the statistical tool to compare sample means reference to specific sample mean? How to use SPSS / Minitab S/W for this purpose? e.g. I want to compare the means of these samples with "Mu"...

05 June 2016 6,446 6 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View