How to chose the best trendline option for biological data?

03 April 2018 2,652 3 View

No title

31 December 2017 1,442 3 View

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

07 August 2017 2,098 13 View

What's the best way to determine the insertion site of a transgene in an Arabidopsis?

Can I use LB and RB boarder primers to amplify the either sides of the trans-gene (cassette)? For this does it necessary to check the copy number of the transgene first?

07 August 2017 5,940 3 View

What are the differences between RNAi and artificial microRNA knockdown plants?

01 February 2017 9,180 0 View

How TOR regulate the autophagy in plants (Arabidopsis)?

31 December 2016 6,924 3 View

What is next, after gathering data from molecular biology experiment? How do you approach data analysis and interpretation?

31 December 2016 1,585 3 View

How to make a cDNA library for protein protein interaction (Y2H)?

07 August 2016 9,340 3 View

How over expression of receptor protein affects in vivo (plants)?

How over expression of receptor protein affects in vivo (plants)? (biochemically) using ectopic promoters / interact protein etc.....

06 July 2016 3,963 1 View

How to compare sample means reference to specific sample mean?

What is the statistical tool to compare sample means reference to specific sample mean? How to use SPSS / Minitab S/W for this purpose? e.g. I want to compare the means of these samples with "Mu"...

05 June 2016 6,446 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View