How TOR regulate the autophagy in plants (Arabidopsis)?
Since tor mutants are embryo lethal, what mutants have been used so far for studying autophagy in plants?
@ Akila,
You are right. Disruption of the TOR gene is lethal and causes an early block in embryo development, impeding the analysis of TOR function in plants. So, people try not to knock-out (no expression) the TOR gene for study. Instead, one way to go by is to overexpress or 'knock-down' (with decreased expression, ex using RNAi) the gene for study.
**See P.2 of the attached paper with yellow highlights.
"Disruption of the TOR gene is lethal and causes an early block in embryo development, impeding the analysis of TOR function in plants. Previous research with transgenic Arabidopsis plants with increased or decreased TOR expression level [40,41] showed that growth of root and shoot was correlated with TOR expression level, indicating a role in growth regulation."
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