How to chose the best trendline option for biological data?

03 April 2018 2,652 3 View

Do plants have similar PI3K-Akt signaling pathway?

09 October 2017 3,576 1 View

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

07 August 2017 2,098 13 View

What's the best way to determine the insertion site of a transgene in an Arabidopsis?

Can I use LB and RB boarder primers to amplify the either sides of the trans-gene (cassette)? For this does it necessary to check the copy number of the transgene first?

07 August 2017 5,940 3 View

What are the differences between RNAi and artificial microRNA knockdown plants?

01 February 2017 9,180 0 View

How TOR regulate the autophagy in plants (Arabidopsis)?

31 December 2016 6,924 3 View

What is next, after gathering data from molecular biology experiment? How do you approach data analysis and interpretation?

31 December 2016 1,585 3 View

How to make a cDNA library for protein protein interaction (Y2H)?

07 August 2016 9,340 3 View

How over expression of receptor protein affects in vivo (plants)?

How over expression of receptor protein affects in vivo (plants)? (biochemically) using ectopic promoters / interact protein etc.....

06 July 2016 3,963 1 View

How to compare sample means reference to specific sample mean?

What is the statistical tool to compare sample means reference to specific sample mean? How to use SPSS / Minitab S/W for this purpose? e.g. I want to compare the means of these samples with "Mu"...

05 June 2016 6,446 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View