How to make a cDNA library for protein protein interaction (Y2H)?

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How to chose the best trendline option for biological data?

I see MS Excel has several trend-line options; linear, logarithmic, polynomial, exponential, and power functions. What is the basis / logic of selecting these functions for biological data. For...

03 April 2018 2,835 3 View

Is there any significant difference between -20°C and -80°C stored biological samples?

Is there any significant difference between -20°C and -80°C stored biological samples (i.e. Plant tissues for proteins, mRNA and metabolite extraction)?

31 December 2017 1,702 3 View

Do plants have similar PI3K-Akt signaling pathway?

Do plants have similar PI3K-Akt signaling pathway? What proteins involve?

09 October 2017 3,765 1 View

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

07 August 2017 2,267 13 View

What's the best way to determine the insertion site of a transgene in an Arabidopsis?

Can I use LB and RB boarder primers to amplify the either sides of the trans-gene (cassette)? For this does it necessary to check the copy number of the transgene first?

07 August 2017 6,096 3 View

What are the differences between RNAi and artificial microRNA knockdown plants?

What are the differences between RNAi and artificial microRNA? is there any advantage of any over other type in creating mutants? How to design the cassette for RNAi and microRNA constructs for...

01 February 2017 9,329 0 View

How TOR regulate the autophagy in plants (Arabidopsis)?

How TOR regulate the autophagy in plants (Arabidopsis)? Since tor mutants are embryo lethal, what mutants have been used so far for studying autophagy in plants?

31 December 2016 7,077 3 View

What is next, after gathering data from molecular biology experiment? How do you approach data analysis and interpretation?

Today molecular biology researchers allow generating massive quantities of multiple types of data with Nextgeneration sequencing, qPCR, microRNA, and Proteomics in less time. For an example, if...

31 December 2016 1,750 3 View

How over expression of receptor protein affects in vivo (plants)?

How over expression of receptor protein affects in vivo (plants)? (biochemically) using ectopic promoters / interact protein etc.....

06 July 2016 4,119 1 View

What may be the reason that ABA deficient mutants has high chlorophyll content?

What may be the reason that ABA deficient mutants (aba2 / aba3) have high chlorophyll content comparing to WT. Any scientific paper available regarding this?

05 June 2016 4,692 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Guetouache Mourad

See the attached files

Saleh Alkarim

Hello

Also these books will help you

Good Luck

http://www.worldcat.org/title/advanced-genetic-analysis-genes-genomes-and-networks-in-eukaryotes/oclc/258074678/editions?editionsView=true&referer=br

http://blog.addgene.org/tips-for-screening-with-yeast-two-hybrid-systems

http://link.springer.com/book/10.1007%2F978-1-59259-919-6

Akila Wijerathna Yapa

Thank you Prof. Mourad