I am getting positive sensogram with blank buffer. What could be potential reason and how can i reduce the same?

Hi, I am using Biacore T200 after FC2-1 with blank buffer i am observing positive sensogram. Please note their running buffer and blank buffer is same in my case. Why is blank buffer (HBSP+ + 1%...

31 December 2018 1,424 2 View

Immobilization issue of biotinylated protein on SA chip Biacore T200 ?

Hi, I am trying to immobilize biotinylated protein to SA chip using biacore T200. The immobilization levels we are getting are low (800-1500RU). Our aim is to immobilize protein upto 4000-5000 RU....

08 September 2018 4,029 4 View

How much protein can we immobilize on SA Chip (BIACORE)?

Hi, I am trying to immmobilize biotinylated Protein on SA chip (BIACORE). However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or...

07 August 2018 1,080 0 View

How to Immobilizing Biotinylated Protein on SA CHIP using Biacore T200?

Hi, I am trying to immmobilize biotinylated Protein on SA chip. However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or HEPES, protein...

07 August 2018 3,378 0 View

From where can i buy GLC16 and SUM149 cell line?

10 November 2015 2,304 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View