Gautam Agarwal

6 Questions 5 Answers 0 Followers

Questions related from Gautam Agarwal

I am getting positive sensogram with blank buffer. What could be potential reason and how can i reduce the same?

Hi, I am using Biacore T200 after FC2-1 with blank buffer i am observing positive sensogram. Please note their running buffer and blank buffer is same in my case. Why is blank buffer (HBSP+ + 1%...

01 January 2019 1,432 2 View

Immobilization issue of biotinylated protein on SA chip Biacore T200 ?

Hi, I am trying to immobilize biotinylated protein to SA chip using biacore T200. The immobilization levels we are getting are low (800-1500RU). Our aim is to immobilize protein upto 4000-5000 RU....

09 September 2018 4,036 4 View

How much protein can we immobilize on SA Chip (BIACORE)?

Hi, I am trying to immmobilize biotinylated Protein on SA chip (BIACORE). However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or...

08 August 2018 1,085 0 View

How to Immobilizing Biotinylated Protein on SA CHIP using Biacore T200?

Hi, I am trying to immmobilize biotinylated Protein on SA chip. However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or HEPES, protein...

08 August 2018 3,396 0 View

From where can i buy GLC16 and SUM149 cell line?

From where can i buy GLC16 and SUM149 cancer cell line? thanks Gautam

11 November 2015 2,334 2 View

What are the culture conditions for beta-TC6 cells?

Hi, whats is the split ratio, when and how frequently we need to split. How much to seed in T25 or T75? Regards Gautam

07 July 2015 3,615 2 View