From where can i buy GLC16 and SUM149 cell line?

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I am getting positive sensogram with blank buffer. What could be potential reason and how can i reduce the same?

Hi, I am using Biacore T200 after FC2-1 with blank buffer i am observing positive sensogram. Please note their running buffer and blank buffer is same in my case. Why is blank buffer (HBSP+ + 1%...

31 December 2018 1,447 2 View

Immobilization issue of biotinylated protein on SA chip Biacore T200 ?

Hi, I am trying to immobilize biotinylated protein to SA chip using biacore T200. The immobilization levels we are getting are low (800-1500RU). Our aim is to immobilize protein upto 4000-5000 RU....

08 September 2018 4,051 4 View

How much protein can we immobilize on SA Chip (BIACORE)?

Hi, I am trying to immmobilize biotinylated Protein on SA chip (BIACORE). However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or...

07 August 2018 1,109 0 View

How to Immobilizing Biotinylated Protein on SA CHIP using Biacore T200?

Hi, I am trying to immmobilize biotinylated Protein on SA chip. However I can bairely immobilize ~1000 RU, with time and flow rate. Following are the condition. Buffer phosphate or HEPES, protein...

07 August 2018 3,417 0 View

What are the culture conditions for beta-TC6 cells?

Hi, whats is the split ratio, when and how frequently we need to split. How much to seed in T25 or T75? Regards Gautam

06 July 2015 3,633 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View