What is the best method for miRNA expression analysis in Rat hippocampus?

Hello to all. I am performing a thesis on Cognitive disorder modeling in rats and the effect of Pharmacological Treatment on the disease and the expression of certain miRNAs. I am seeking...

14 October 2020 6,690 1 View

Is there any need to Do PCR After RT-PCR?

Hello. I would be thankful if anyone could answer my question. We are Analyzing the expression of a certain gene in our research. We have extracted all the mRNAs in the cell and now I was...

22 July 2020 768 4 View

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As you may know, blocking the receptor binding domain (RBD) of novel coronavirus could be very effective to prevent more severe effects of the inflammation. So, can a BsAb co-targeting CD8+ t...

09 April 2020 8,900 1 View

Where can I find secondary data on the relationship between organizational change cynicism and organizational commitment?

Where can I find secondary data on the relationship between organizational change cynicism and organizational commitment? So that I can re-analyze it for my dissertation. Of course credit will be...

05 February 2020 4,794 2 View

Can anyone introduce a review paper about different methods of optical force calculation?

Hi, Maxwell Stress Tensor (MST) method is usually used to calculate the optical force exerted on an object. However, my question is about the applicability range of this method. When do we use...

28 January 2019 4,261 3 View

How to purify genomic DNA following DpnI treatment?

02 October 2016 9,040 3 View

How can I find Building stone like travertine and marble?

Hello dear friends. I want to know how can I find Building stones like Travertin and Marble according Remote Sensing and geochemistry anomaly or ....Actually in Iran we find Building Stones by...

06 August 2016 8,735 3 View

Hi, Does anyone know a suitable media for streptococcus zooepidemicus which does not contain sugar?

Conventional media for this bacteria is BHI but it contains glucose

16 September 2015 6,950 3 View

What is the best tissue culture (T25 and T75 flask) provider?

I am planning to place a large order and need a good source for T25 and T75 flasks. I am looking for good quality for reasonable price. Thank you.

26 April 2015 9,470 6 View

How to calculate dimensionless equilibrium constant (Ke) for calculation of the thermodynamic parameters of the adsorption by van't Hoff equation?

While many authors applied the van't Hoff equation for the calculation of the thermodynamic parameters of the adsorption, but there is widespread misuse of adsorption isotherm constants or...

01 January 1970 6,698 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View