Our lab has always ordered the goat anti mouse highly cross preadsorbed secondary antibodies from Thermo but we find that there is a lot of batch to batch variation in terms of cross reactivity with rat. This is a problem for us as one of our labs favorite antibodies is a rat tubulin antibody that we like to combine with mouse antibodies. While a work around has been to first incubate mouse primary, then mouse secondary, then the other primary antibodies followed by their secondary antibodies, it would be preferable to find a reliable source of mouse secondaries that do not show cross reactivity with rat. Any recommendations?
Hi Joyce C.M. Meiring
Have you tried using Alexa Fluor from Thermo?
We use Alexa Fluor secondary antibodies for all types of Immunocytochemistry and Immunofluorescence in in-vitro cells and in-vivo tissue sections. It works very well for imaging and has no cross reactivity with rat specific primary antibodies.
Please find the link for your reference.
1. https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG-H-L-Secondary-Antibody-Recombinant-Polyclonal/A28175
Thanks,
Saroj Kumar Khan Samir Ranjan Panda
Hi Saroj and Samir,
I have been experiencing very variable levels of cross reactivity with the highly cross preadsorbed mouse thermo antibodies so I was looking for a better alternative.