What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

11 January 2021 8,775 10 View

How to analyze Longitudinal Pre/Post different subjects?

I have 6 years of pre program data for two schools and I have 6 years of Post program data for the same two schools. The issue is that the POST data is not in the same years and the PRE data. I'm...

05 January 2021 4,587 5 View

Independent T tests a priori with MANOVA?

I am conducting a causal-comparative study for my dissertation, and am under quality review. The reviewer told me that I should prepare for post hoc testing by conducting independent t tests a...

16 October 2019 5,465 3 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Any advice on why my Her2 protein is not being coated to my amine plate?

I coat 1 ug/mL of the Her2 protein with 100 uL 100mM carbonate buffer to my flat bottomed well and have only had a positive result once out of 8 times. I have tried coating at 4 deg C overnight,...

30 November 2016 3,720 5 View

How can criminalizing prostitution and sex trade aid in healthcare intervention for such victimization and abuse?

07 July 2016 4,742 2 View

Are human B and T cells adherent or suspension cells in culture? I think they're sticking to the bottom of my plate. Possible?

26 April 2016 2,726 6 View

Does anyone have any interesting references on memory distortion and eyewitness evidence?

02 April 2015 1,285 4 View

Any suggestions to get the characteristic red pigment expressed by Microsporum gallinae?

Microsporum gallinae ATCC 90749; received strain from ATCC and have grown on Sabouraud dextrose agar at various temperatures.

03 April 2014 249 2 View

Problem with Touchdown PCR for DRD2 SNP.

I'm doing analysis on a DRD2 SNP for ADHD patients, with Touchdown PCR and electrophoresis. For the last 3 weeks I had to work on another PCR machine and now the Touchdown PCR doesn't work at all...

07 February 2013 6,119 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View