What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

11 January 2021 8,775 10 View

How to analyze Longitudinal Pre/Post different subjects?

I have 6 years of pre program data for two schools and I have 6 years of Post program data for the same two schools. The issue is that the POST data is not in the same years and the PRE data. I'm...

05 January 2021 4,587 5 View

Independent T tests a priori with MANOVA?

I am conducting a causal-comparative study for my dissertation, and am under quality review. The reviewer told me that I should prepare for post hoc testing by conducting independent t tests a...

16 October 2019 5,465 3 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Any advice on why my Her2 protein is not being coated to my amine plate?

I coat 1 ug/mL of the Her2 protein with 100 uL 100mM carbonate buffer to my flat bottomed well and have only had a positive result once out of 8 times. I have tried coating at 4 deg C overnight,...

30 November 2016 3,720 5 View

How can criminalizing prostitution and sex trade aid in healthcare intervention for such victimization and abuse?

07 July 2016 4,742 2 View

Are human B and T cells adherent or suspension cells in culture? I think they're sticking to the bottom of my plate. Possible?

26 April 2016 2,726 6 View

Does anyone have any interesting references on memory distortion and eyewitness evidence?

02 April 2015 1,285 4 View

Any suggestions to get the characteristic red pigment expressed by Microsporum gallinae?

Microsporum gallinae ATCC 90749; received strain from ATCC and have grown on Sabouraud dextrose agar at various temperatures.

03 April 2014 249 2 View

Problem with Touchdown PCR for DRD2 SNP.

I'm doing analysis on a DRD2 SNP for ADHD patients, with Touchdown PCR and electrophoresis. For the last 3 weeks I had to work on another PCR machine and now the Touchdown PCR doesn't work at all...

07 February 2013 6,119 4 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Which factors contribute to instructional resilience in HE?

If you are teaching in Higher Education, please consider participating in our study on Instructional Resilience during Emergency Remote Teaching. We want to learn about which factors have...

19 January 2021 6,759 15 View

Can the benefits of resilience be linked to the types of resilience?

Hi All I am trying to obtain journal articles on the benefits on resilience. However, I was thinking - can the types of resilience (physical, emotional, psychological & community...

02 January 2021 7,409 6 View

Why people feel fear and not share their particulars during social or questionnaire survey, especially in Pakistan?

Why people feel fear and not share their particulars during social or questionnaire survey, especially in Pakistan?

01 January 2021 4,782 12 View

Writing participant characteristics in method while being LGBTQI+ sensitive?

I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing. I have a sample of X participants in a...

29 December 2020 9,011 3 View

Is Covid-20 is similar to Covid-19? Or what are the major difference?

Covid-19 Vs Covid-20 Covid-20 fear More strong corona virus

27 December 2020 3,312 8 View

What is your understanding about death ?

Do you think of it as a part of life, as a disease without a cure, as a way of immortalization, as a way of transformation, as a way to freedom..or something else. Is it good or bad? We all know...

22 December 2020 2,041 37 View