What are the advices of encapsulation of proteins using PLGA and double emulsion technique?

01 January 2016 0 8K Report

I am trying to encapsulate a protein hydrolysis using PLGA. the protein loading is very low. if you have an advice, please, share it.

Badges
Science topic

More Ebrahim Elkhtab's questions See All

What is the best concentration of Acetic acid to be added in the manufacturing of mozarella cheese?

Dear professors, I am going to manufacture mozarella cheese with direct acidification method using Acetic acid. what is the best concentration of acetic acid? is there any preferd conditions...

09 October 2017 2,014 0 View

How to dissolve Moringa oliefera extract?

Dear professors, I have an extract from moringa leaves. it is extracted with solvents. my question is how to dissolve this extract in a good way?

08 September 2017 8,898 5 View

Spss mixed Anova equation?

I am looking for the right source to get the equations of Anova by Spss, if you know the right source, please refer it to me.

31 December 2015 4,523 2 View

What is the justification of using 26s primer for identifying yeast?

what is the justification of using 26s primer for identifying yeast?.

09 October 2015 3,341 1 View

What is the mode of action of captopril?

I need to understand the mode of action of captopril as a drug for hypertension

09 October 2015 293 2 View

How do I extract encapsulated peptides?

If you have encapsulated peptides in cyclodextrin and would like to extract these peptides from encapsulation statue, what is the method for this?

03 April 2015 1,896 5 View

How can I fix or refresh the pH electrodes?

does any body have idea about fixing pH electrodes?

02 March 2015 6,700 12 View

Does anybody know the fluorescence of histidine-leucine?

i need to know the fluorescence spectrum of histidine-leucine peptide and for histidine and leucine amino acids

01 February 2015 3,723 3 View

What is the best wave length for measuring the absorbance of peptides during the HPLC run?

What is the best wave length for measuring the absorbance of peptides during HPLC run?

11 December 2014 8,260 14 View

What is the main function of fourier transform infrared spectroscopy for peptides?

See above

11 December 2014 5,386 5 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

11 July 2024 4,645 3 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View