What is the best concentration of Acetic acid to be added in the manufacturing of mozarella cheese?

Dear professors, I am going to manufacture mozarella cheese with direct acidification method using Acetic acid. what is the best concentration of acetic acid? is there any preferd conditions...

09 October 2017 1,852 0 View

How to dissolve Moringa oliefera extract?

Dear professors, I have an extract from moringa leaves. it is extracted with solvents. my question is how to dissolve this extract in a good way?

08 September 2017 8,705 5 View

What are the advices of encapsulation of proteins using PLGA and double emulsion technique?

I am trying to encapsulate a protein hydrolysis using PLGA. the protein loading is very low. if you have an advice, please, share it.

31 December 2015 8,278 0 View

Spss mixed Anova equation?

I am looking for the right source to get the equations of Anova by Spss, if you know the right source, please refer it to me.

31 December 2015 4,381 2 View

What is the mode of action of captopril?

I need to understand the mode of action of captopril as a drug for hypertension

09 October 2015 147 2 View

How do I extract encapsulated peptides?

If you have encapsulated peptides in cyclodextrin and would like to extract these peptides from encapsulation statue, what is the method for this?

03 April 2015 1,755 5 View

How can I fix or refresh the pH electrodes?

02 March 2015 6,491 12 View

Does anybody know the fluorescence of histidine-leucine?

i need to know the fluorescence spectrum of histidine-leucine peptide and for histidine and leucine amino acids

01 February 2015 3,569 3 View

What is the best wave length for measuring the absorbance of peptides during the HPLC run?

What is the best wave length for measuring the absorbance of peptides during HPLC run?

11 December 2014 8,004 14 View

What is the main function of fourier transform infrared spectroscopy for peptides?

See above

11 December 2014 5,250 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View