what are tail anchored proteins
I want to work towards decreasing side effects of a particular class of drugs. Can anyone guide me as to how this can be done.
09 October 2015 3,445 0 View
What is difference between HypoGen and HipHop which are modules for generation of Pharmacophore models? Which method should be used when. Advantages and disadvantages of each.
07 August 2015 8,734 0 View
what does a subgenomic replicon assay mean? I came across this term in various papers but could not understand it. Can someone please explain its meaning in simple language.
07 August 2015 1,554 0 View
I have come across the term truncated N terminal various times in several papers. I want to know the importance of truncation and what it means. Why truncation is used.
07 August 2015 7,402 1 View
What does root mean square deviation(RMSD) mean. How is it useful wehile developing pharmacophore models and QSAR models?
07 August 2015 7,758 0 View
There are various steps in protein preparation. One of them is refinement. Why is it required?
07 August 2015 9,614 0 View
With respect to interaction between a ligand and a protein what does a supramolecular interaction mean?
07 August 2015 3,992 0 View
In protein preparation why is restrained minimization in vacuum needed. What does convergence criteria for heavy atom RMSD mean. What is its importance. What happens if minimization is performed...
07 August 2015 525 0 View
In molecular docking we can perform Molecular dynamics. What are the potential uses and how to interpret results in Molecular dynamics.
07 August 2015 5,942 1 View
what does a vector attribute mean and its importance in Phaermacophore modelling using PHASE provided by Schrodinger?
07 August 2015 7,002 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View
