What alternative product do you use to prepare HL5 culture medium?

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Can someone help with the instrument configuration in Profex using Rietveld Refinement?

I am trying to configure the instrument in Profex but I can't get a chi-squared value below 9.65 for LaB6. This is the configuration of the equipment, which is a Bruker D8 Advance with a Linxeye...

14 July 2024 4,767 1 View

Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

08 July 2024 9,732 1 View

¿ Algún experto en simulación de inversores ?

Simular una topología de inversor para aplicaciones de vehículos eléctricos en el software PSIM

20 June 2024 2,490 0 View

How can I report grams of dry weight or dry sample of a plant extract that was concentrated and then freeze-dried?

for context, they are methanol/water extracts that were concentrated in a rotavapor and then freeze-dried. thank U.

09 May 2024 9,941 3 View

Hello everyone! Would you please help me identifying this marine polychaete ?

It was extracted from a sediment sample in the subtidal zone

19 April 2024 6,725 4 View

Is it wrong to add one more author over the limit imposed by the journal?

Hi, my team and I want to publish a paper. However, the journals we are overseeing for possible submission only accept 3 authors and we are 4. Would it be wrong or prone to desk rejection if we...

16 April 2024 3,945 4 View

Optimal concentration of IL-6 for stimulation?

Hi! I'm trying to stimulate cells with IL-6 (in order to perform a qPCR) but i can't find a proper protocol. Any suggestions?

03 April 2024 379 3 View

¿Cómo ha afectado el impacto del avance de las TIC en el ámbito del trabajo social?

Con esta pregunta se pretende saber los diferentes cambios que ha producido las TIC en estos últimos años en diversos aspectos de la vida relacionados con situaciones cotidianas en el Trabajo Social.

11 March 2024 8,740 2 View

¿Qué recursos son necesarios en Servicios Sociales para tratar de eliminar barreras de acceso laboral en personas con discapacidad?

Con este planteamiento se pretende hacer reflexionar a los lectores de los diferentes planes de actuación, medidas y otros tipos de propuestas que pueden ser implantados en los Servicios Sociales...

10 March 2024 1,481 4 View

¿Qué actuaciones faltan para favorecer una garantía de derechos de las personas en situación de dependencia y/o sus cuidadores?

Con este planteamiento se pretende que los lectores tomen conciencia del enorme problema social que supone en la sociedad andaluza que una persona se encuentre en situación de dependencia. De los...

10 March 2024 6,151 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Caroline Baudson

Hi,

If you have found an answer, I would be glad to hear it because I do not find this product and I want to start cultivating Dictyostelium.

Best regards,

Caroline

Inmaculada Ramírez-Macías

Hi Caroline,

I use neutralised bacteriological peptone from Oxoid (Ref LP0034).

Best regards

Inmaculada

Kishore Bhat

you can use peptone AT (peptic digest of animal tissue). It is same as thiotone