I recently have some problems for my protein transfer as seen in the attached photo. It doesnt look like air bubble trapped but instead seems the membrane or gel was damaged. Can anyone please have any suggestions? Thanks
There could be different causes of such results:
1. The membrane could be insufficiently wetted or dried out before transfer.
2. If you perform transfer in a tank, make sure that the system is cool. Overheating may produce air bubbles trapped inside the sandwich.
3. This result could actually be a result of improper sandwich assembly. Make sure you removed all the air bubbles during the process.
Good luck!
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...
23 February 2021 7,194 4 View
18 February 2021 9,188 3 View
There are two plates with horizontal center crack, one with crack length of a with the other with a+da. The bottoms are fixed while the tops are under the same displacement load. For intuition,...
11 February 2021 9,543 2 View
I am working on one optimization problem. the model with two states, the transition between the two states based on one threshold value (L = 5, which L< 5 -> Normal state, L== 5 ->...
11 February 2021 4,045 3 View
Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...
24 January 2021 9,912 5 View
I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.
17 January 2021 1,123 3 View
I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).
14 January 2021 3,805 2 View
I just developed a new theory on electromagnetic and plan to publish in a Journal. The findings are purely through strict mathematical derivation without any disputable assumptions and also...
13 January 2021 1,558 5 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
02 March 2021 7,082 3 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...
01 March 2021 6,200 3 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...
27 February 2021 1,233 1 View