Chen Hao

5 Questions 1 Answers 0 Followers

Questions related from Chen Hao

KEAP1 Western blot double bands?

Hi guys, Im using KEAP1 antibody from CST (D6B12) or santa cruz (G-2) and i found both antibodies give close double bands at the expected MW (~70 kda). The example blot images on CST and SC...

22 December 2021 3,101 1 View

Westernblot transfer troubleshooting?

I recently have some problems for my protein transfer as seen in the attached photo. It doesnt look like air bubble trapped but instead seems the membrane or gel was damaged. Can anyone please...

07 November 2021 1,977 1 View

Has anyone had the experience of producing poly-Arg phosphopeptide?

Hello, Im trying to make phosphopeptide conjugated to poly-Arg for better cell permeability. Im wondering if the positively charged Arg will form stable intra-molecule interaction with negatively...

20 June 2020 5,405 0 View

Question about immunoprecipitation results

Hi guys! I am doing immunoprecipitation to verify the interaction between two proteins. One protein is His-tagged and the other is HA-tagged. When I use nickel beads to pull down the His-tagged...

14 August 2017 7,031 3 View

Why we assume homogeneity of variance in unpaired t-test?

If we violate this assumption and still operate t-test, what will happen? Will it affect the p-value?

31 March 2017 5,484 4 View