Pr Mei

6 Questions 15 Answers 0 Followers

Questions related from Pr Mei

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

19 July 2024 9,148 2 View

Western blot showed distorted band, reverse 'U' shape, low MW protein?

This is 12% SDS PAGE gel and the MW of this protein is around 20 kDa. Running: 80V 20min; 110V 2h30min Transfer: 100V 1h20min I wonder this might be due to the gel over-heated during the running?

05 January 2024 7,375 0 View

What causes the faint and smeared bands in agarose gel electrophoresis?

The cDNA was amplified by Q5 and PCR product was analyzed by agarose gel electrophoresis. Would cDNA template degradation cause such smeared or smiling bands?

14 December 2023 3,007 12 View

Will endogenous and over-expressed protein level show different band size in western blot?

Pull down assay showed a discrepancy of band size between endogenous and over-expressed stable clones. Left: Endogenous Right: OE

31 October 2023 623 6 View

Why did DNA agarose gel electrophoresis show smearing band?

I used KAPA hifi pcr kit to linearize the plasmid for in-fusion cloning. Following the protocol, the DNA template amount was 5 ng and I already diluted it 100x. Annealing temperature: 66 69. The...

30 May 2023 5,212 5 View

What is the difference between Lipofectamine 2000 and Lipofectamine 3000 for stable transfection of Lentivirus?

What is the difference between Lipofectamine 2000 and Lipofectamine 3000 for stable transfection of Lentivirus? I was told that Lipofectamine 3000 is more toxic to the cells with higher...

13 December 2022 7,004 4 View