Hello all! So I am looking at very small proteins (~15kD) and just switched to the Bis-Tris system. I poured my own 12% gel, made up MOPS running buffer, and went ahead and bought the NuPage sample buffer. Typically to prepare samples to be loaded onto a gel, we add 3x sample buffer with BME and benzonase directly to the cells. For the tris-gly system this sample preparation has been working great. Unfortunately, when preparing my cells in the 4x NuPage sample buffer with the added BME and benzonse, I had a bit of snot and my blot did not turn out well. Any thoughts on how to prepare cell lysates for the bis-tris system is much appreciated!