Kimberly Edicha

4 Questions 2 Answers 0 Followers

Questions related from Kimberly Edicha

Why is my bis-tris gel not resolving well?

Hello all! I am struggling to understand why my bis-tris gel is not resolving well. There is not a uniform band forming in the lanes causing the gel to turn completely blue. I am using this...

21 December 2021 2,897 1 View

Using Bis-Tris Gels for the First Time?

Hello all! So I am looking at very small proteins (~15kD) and just switched to the Bis-Tris system. I poured my own 12% gel, made up MOPS running buffer, and went ahead and bought the NuPage...

09 November 2021 4,127 1 View

How do you reduce smearing on a 4-20% gradient gel?

Hello! I am getting some smearing appear on these 4-20% gradient gels. I loaded 20ug of protein each time. I am seeing my bands fine but the rest of the gel is a bit of a mess. I have attached an...

28 July 2021 2,553 3 View

Optimal Lysis Buffer for Whole Cell Lysate WB?

I have samples of KO membrane proteins in Hela cells that I am trying to view on WB and am having trouble determining the best lysis buffer to do so. We have tried 0.5% Np-40, 10mM Hepes pH 7.4,...

22 July 2021 9,163 2 View