Hi,
I am doing a freelance project on bone defects treated with control and treated bone sponge- so I know little about the actual study and bone is not my forte in science.
I'm told the sections were stained with Masson's trichrome. The bone is solid deep blue and "everything else" is red nor lighter blue. I'm interested in what "everything else" is. I can identify the bone marrow and fatty cells by the holey appearance. Can anyone help me identify anything else in the these slides?
Thank you! Thank you! Thank you!
Hello!!
From my not very long experience, as you say, we can see the adypocites surrounding the bone marrow.
Commonly, the blue stands for collagen, which in the bone tissue normally is type 1, although at some levels of ossification we could see type 2 collagen.
I suppose that the cells within all the blue structures might be osteocytes.
Because it is bone marrow, the fibers that sorround it will be type 3 collagen, and would be positive with PAS because of the proteoglycans that sorround them or with silver staining.
I would love to answer you more specifically, but the image is not very close. I have achieved to see some Megakaryocites or and some monocytes or macrophages. Because you have said that it was a reparation tissue, i would assume that it is a macrophage. If you have a bigger picture, please send it to me.
Wish you the best!!!
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