Hello all,
I have used HEPES in the past to maintain my cells outside of CO2 incubators for extended periods of time, however some of my recent work would best be performed if the bicarbonate buffering system was not involved and simply relying on HEPES to maintain pH.
I have grown some HeLa cells in this medium (standard EMEM w 10% HIFC, Penstrep and NEAA) and noticed cells will proliferate but demonstrate a 'stretched' morphology, not the more classical appearance they usually have.
Does this imply the bicarbonate present in the media also influences cell morphology? If so, do we think that the absence of said bicarbonate would influence cell metabolism?
Any info regarding this is appreciated!
Dear Craig
We know that much that sodium bicarbonate is a buffer used to stabilize the pH. As far as I understand there is no independent study to show the effect of sodium bicarbonate on this specific cell line. CO2 that was produced by the cells will affect the pH of the medium (make it more acidic) but HEPES sometimes are being used to prevent that. In your case, I believe the slight change in pH and acidity can have a major impact on the morphology of the cells especially when you add HEPES and remove bicarbonate to introduce them to an open environment. I believe you need the bicarbonate to make it more stable to change of pH. I hope I could help you with that.
Best
Mahyar
It is well stablished that bicarbonate present in the media influences cell growth and cell morphology in cancer cells. This may be due to its role in cell metabolism. more research is still needed for clarification this role.
Hi Craig,
Which is the percentage that you use of HEPES in your media?
- Badges
- Science topic